发明名称 |
MEDIEN FÜR DIE ISOLIERUNG UND STABILISIERUNG VON ZELLEN |
摘要 |
Viable microvessel endothelial cells are isolated from tissue in high yield by enzymatic digestion of tissue containing the cells using as a medium for the digestion, an enzyme composition in a physiologically acceptable aqueous electrolyte solution of pH 6.5 to pH 8.0 consisting essentially of sodium, potassium, magnesium, chloride, acetate, and gluconate ions, and having an osmolarity of about 270 mOsmol/L to 310 mOsmol/L. The electrolyte solution when mixed with a blood component is effective for stabilizing viable animal cells for periods of up to 4 hours. |
申请公布号 |
DE69425707(T2) |
申请公布日期 |
2000.12.28 |
申请号 |
DE1994625707T |
申请日期 |
1994.01.11 |
申请人 |
BAXTER INTERNATIONAL INC., DEERFIELD |
发明人 |
HU, B.;NORTHUP, J.;MYERS, E.;QUIJANO, C. |
分类号 |
C12N5/06;A01N1/02;A61F2/06;A61L27/00;A61L27/50;C12Q1/24;(IPC1-7):C12Q1/24 |
主分类号 |
C12N5/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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