发明名称 |
TECHNIQUES FOR PIERCING SPECIFIC SITE OF CELL |
摘要 |
<p>A technique for controlling membrane denaturation reactions other than physical shear force was developed. For example, the present invention provides, a method for causing membrane disruption at a specific site by reacting a stimulus such as light with a compound that is activated by the stimulus, where the reaction occurs on a membrane such as a biomembrane. It also provides a membrane structure such as cells in which a specific site has been disrupted, which are obtained by the present method. Introduction of substances such as genes also became possible by using this membrane structure. Further provided is a membrane-destroying member for disrupting a membrane at a specific site. Thus, the present invention enabled, for example, easy membrane penetration using components constituting microelectrodes, micromanipulators, and microinjectors, which were conventionally hardly usable in penetrating cell membranes.</p> |
申请公布号 |
EP1063287(A1) |
申请公布日期 |
2000.12.27 |
申请号 |
EP19990939150 |
申请日期 |
1999.03.12 |
申请人 |
KARUBE, ISAO;SAITOH, TAKASHI;CENTER FOR ADVANCED SCIENCE AND TECHNOLOGY INCUBATION, LTD |
发明人 |
KARUBE, ISAO;SAITOH, TAKASHI |
分类号 |
C12M3/00;C12N13/00;C12N15/89;(IPC1-7):C12M1/00;C12N5/06 |
主分类号 |
C12M3/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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