A method for identifying antisense oligonucleotides that bind a target mRNA

摘要

A method for identifying an antisense oligonucleotide capable of binding to a target mRNA, which comprises contacting the target mRNA with each member of an oligonucleotide library separately under hybridisation conditions, removing unhybridised material and determining which member or members hybridise. The oligonucleotide library comprises a plurality of distinct nucleotide sequences of a predetermined common length, and wherein each nucleotide sequence comprises a known sequence of 4 to 5 bases and all possible combinations of the known sequence are present in the library.