| 摘要 |
<p>A method for the efficient production of a population of cells containing a heterologous DNA of interest at low copy number comprises: providing host cells (e.g., plant cells) for transformation; and then transforming the host cells with a DNA construct comprising, in the 5' to 3' direction, a first matrix attachment region, a DNA of interest, and a second matrix attachment region, wherein the first and second matrix attachment regions are in inverted orientation with respect to one another; where the DNA of interest is incorporated into the host cells at low copy number as compared to that which would occur if the first and second matrix attachment regions were in direct orientation with respect to one another. The DNA of interest may comprise, in the 5' to 3' direction, a transcription initiation region functional in the host cell, and a heterologous DNA segment to be transcribed in the host cell. The transforming step is preferably a direct transformation step, such as electroporation, microprojectile bombardment or the like. Where plant cells are employed, shoots and/or roots, or whole plants, can be regenerated from the transformed cells in accordance with known techniques.</p> |
