| 摘要 |
<p>The present invention provides methods and kits for assaying homocysteine in biological samples. One method utilizes the enzymes methionine synthase and serine hydroxymethyltransferase sequentially to produce 3H2O in proportion to the amount of hCys in the original sample. Another method utilizes the enzyme methionine synthase and then the enzymes serine hydroxymethyltransferase, methylene tetrahydrofolate dehydrogenase and formyl tetrahydrofolate dehydrogenase, to produce NADPH in proportion to the amount of hCys in the original sample. The figure shows metabolic pathways for the formation and breakdown of hCys.</p> |
