| 摘要 |
<p>Expression vectors for animal cells comprising any combination of the following techniques: (1) ligating a novel posttrancriptional regulatory sequence to the 3'-downstream of an SRα promoter enhancer sequence (hereinafter referred to as SRα sequence), which is a known highly efficient transcriptional regulatory sequence, to thereby optimize the transcriptional regulation of mRNA transcribed from the promoter; (2) partly modifying bases in the core promoter domain in the SRα sequence to thereby elevate the transcriptional efficiency and delete the ability to self-replicate; (3) modifying the ATG sequence located immediately downstream the promoter in the SRα sequence, which has been optionally modified, to thereby elevate the transcriptional efficiency; and (4) inserting a sequence concerning the nontranscriptional spacer domain sequence of a ribosomal RNA gene into the upstream of the SRα sequence, which has been optionally modified, via a novel liner sequence to thereby elevate the expression efficiency.</p> |
