发明名称 |
METHOD FOR REVEALING PLAGUE MICROBE IN FLEAS |
摘要 |
medical microbiology. SUBSTANCE: invention is intended for bacteriologic diagnostics of objects suspicious of being plague infection transmission entities. For that, flea suspension in 0.9% sodium chloride solution is prepared and divided into three parts. One part (0.8-1.2 ml) is examined in polymerase chain reaction (0.3-0.4 ml) to detect gene pFra, pCad, pPst sequences. Second part of suspension is sowed (each time 0.1-0.15 ml) on dense Jackson-Berrose nutrient medium with gentiane violet (0.02-0.03 mcg/ml) to detect feature PGm+ (determined by chromosome gene sequence). Third part of flea suspension is sowed (each time 0.1-0.15 ml) on Chottinger agar with gentiane violet (0.02-0.03 mcg/ml) to determine sensitivity to antibiotics using paper disk technique. EFFECT: accelerated plague microbe revealing procedure and enabled determination of virulence of microbes. 3 ex
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申请公布号 |
RU2160781(C1) |
申请公布日期 |
2000.12.20 |
申请号 |
RU19990115582 |
申请日期 |
1999.07.13 |
申请人 |
STAVROPOL'SKIJ NAUCHNO-ISSLEDOVATEL'SKIJ PROTIVOCH;UMNYJ INST |
发明人 |
BRJUKHANOVA G.D.;BRJUKHANOV A.F.;EVCHENKO JU.M.;GRIZHEBOVSKIJ G.M.;EREMENKO E.I.;SHCHEDRIN V.I.;MEZENTSEV V.M. |
分类号 |
C12Q1/04;G01N33/53;(IPC1-7):C12Q1/04 |
主分类号 |
C12Q1/04 |
代理机构 |
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