发明名称 |
Process and kit for fragment cloning |
摘要 |
The subject matter of the invention concerns three cosmid vectors which are suitable for fragment cloning of a size between 7 and 36 kb. These vectors consist of an E. coli ColE1 replica, an ampicillin resistance gene, a multiple cloning cassette, cos sites for in vitro packaging with the lambda packaging extracts as well as fragments from the genome of the bacteriophage lambda. The lambda sequences were selected in a way to prevent lytic processes, vector instabilities (deletions) and unwanted recombination events between lambda DNA and the fragment to be cloned. Depending on the length of the lambda fragment inserted in the corresponding vector heterologous fragments of different lengths can be cloned.
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申请公布号 |
US6162633(A) |
申请公布日期 |
2000.12.19 |
申请号 |
US19980193488 |
申请日期 |
1998.11.17 |
申请人 |
METZLER, THOMAS;SOBEK, HARALD;REICHHUBER, ROLF |
发明人 |
METZLER, THOMAS;SOBEK, HARALD;REICHHUBER, ROLF |
分类号 |
C12N15/09;C12N1/04;C12N1/21;C12N15/70;C12Q1/68;C12R1/19;(IPC1-7):C12N1/20 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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