| 摘要 |
PURPOSE:To obtain a microorganism sensor capable of measuring sulfate ion till a low concentration by a simple operation. CONSTITUTION:First, Thiobacillus ferrooxidans E-15 strain as an immobilized bacterium is inoculated to 9K liquid medium such as Silverman's liquid medium, subjected to shaking culture at 30 deg.C for three days, the medium is centrifuged at low speed and centrifuged at 8,000rpm for 10 minutes to collect cells. Then the collected cells are once washed with diluted sulfuric acid adjusted to pH 2, then twice with 5mM glycine-hydrochloric acid buffer solution (pH 2.7) at 4 deg.C and the prepared suspension of the cells is filtered with a nitrocellulose filter by suction and the filter to which the cells are stuck is used as an immobilized bacterium film 8. Then, the immobilized bacterium film 8 is enclosed with a nylon net 9 having 200 meshes and fixed to the top of an oxygen electrode 1 equipped with a gas passable Teflon membrane 6 with an O ring 7 to prepare a bacterial electrode. A resistance (3) of 5KOMEGA is connected to the bacterial electrode in parallel and the resistance is connected to a recorder 2. |
