发明名称 Processes for preparing and using immobilized lipases
摘要 PCT No. PCT/EP96/02587 Sec. 371 Date Dec. 29, 1997 Sec. 102(e) Date Dec. 29, 1997 PCT Filed Jun. 14, 1996 PCT Pub. No. WO97/01632 PCT Pub. Date Jan. 16, 1997An amphiphilic enzyme is immobilized by preparing an emulsion containing a continuous hydrophobic phase and a dispersed aqueous phase containing the enzyme and a carrier for the enzyme, and removing water from the dispersed phase until this phase turns into solid enzyme coated particles. The enzyme is preferably a lipase, and the immobilized lipase can be used for reactions catalyzed by lipase such as interesterification of mono-, di- or triglycerides, de-acidification of a triglyceride oil, or removal of phospholipids from a triglyceride oil when the lipase is a phospholipase. The aqueous phase may contain a fermentation liquid, an edible triglyceride oil may be the hydrophobic phase, and carriers include sugars, starch, dextran, water soluble cellulose derivatives and fermentation residues. A substance to be processed such as triglycerides, diglycerides, monoglycerides, glycerol, phospholipids or fatty acids may be in the hydrophobic phase.
申请公布号 US6162623(A) 申请公布日期 2000.12.19
申请号 US19970983085 申请日期 1997.12.29
申请人 LIPTON, DIVISION OF CONOPCO, INC. 发明人 GROTE, MARTIN ROGER;GEURTSEN, JOHAN PAUL;VAN PUTTE, KAREL PETRUS
分类号 C12N9/16;C12N11/02;C12N11/08;C12N11/10;C12P7/64;(IPC1-7):C12P7/64;C12N11/12 主分类号 C12N9/16
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