IGMMONOCLONAL ANTIBODY AGAINST S-PROTEIN OF HEPATITIS B VI RUS AND COMPOSITION FOR THE DIAGNOSIS OF HEPATIC-DISEASE COMPRISING SAME

摘要

PURPOSE: Provided is IgM monoclonal antibody which shows specificity to X protein of hepatitis B virus and is useful in detecting cancer cells, and measuring the level of antigens and antibodies in blood. Also, hepatitis diagnosis composition that contains the monoclonal antibody is provided. CONSTITUTION: IgM monoclonal antibody is obtained by the next steps of: i) inserting SP2/0 X-02M (KCTC 0346BP) into a rat and obtaining spleen cells after 4 weeks; ii) mixing mouse spleen cells with myeloma cells and centrifuging the cells to obtain cell lysates; iii) facilitating the growth of hybridoma in HT medium; iv) selecting hybridoma cells which produces IgM monoclonal antibody through ELISA (Enzyme Linked Immunosorbent Assay); v) separating and purifying anti-X monoclonal antibody IgM; and vi) measuring the absorbency of anti-X monoclonal antibody IgM.