POTENTIATED NUCLEIC ACID AMPLIFICATION METHOD

摘要

A potentiated method for amplifying a specific nucleic acid which comprises, in analyzing a specific nucleic acid in a sample anticipated as containing the specific nucleic acid, using a specific RNA sequence in the sample as a template; thus forming a double-stranded DNA containing sequences complementary to and homologous with the specific RNA sequence and having a promoter sequence whereby these sequences can be transcribed; forming an RNA transcription product by using RNA polymerases; and, in an RNA amplification process wherein the RNA transcription product further serves as a template of a double-stranded DNA, adding inosine triphosphate, in addition to adenosine triphosphate, uridine triphosphate, cytidine triphosphate and guanosine triphosphate, as substrates for the RNA polymerases, thereby elevating the efficiency in the amplification reaction.