| 摘要 |
<p>The present invention provides pH sensitive cyanine dyes having the structure (I) wherein X and Y are independently selected from >C(C1-C4alkyl)2, sulfur and oxygen; R?1 and R2¿ are independently selected from H, CH¿2?NH2, SO3; phosphate, phosphonate, quaternary ammonium, NO2, (CH2)qCOOH, NCS, CH2NH-COR?7¿ where R7 is C1-C20 straight or branched alkyl and -(CH2) q-COOH where q is an integer from 0-10; R3 is H or -L-P where L is selected from C¿1?-C20 straight or branched alkyl optionally containing 0, 1 or 2 unsaturated groups selected from alkenyl, alkynyl and aryl, and P is selected from a reactive group, H, C1-C20 straight or branched alkyl, SO3?-, NH¿2, quaternary ammonium, CH2NH-COR8 where R8 is C1-C20 straight or branched alkyl, -(CH2)m-COOH, NHR9 where R9 is C1 to C20 straight or branched alkyl or COOH; n is an integer from 0-3; p and r are independently 0, 1, 2, 3 or 4 and where p and/or r is greater than 1, each R1 and each R2 may be different, and m is an integer from 1-10, and salts and protonated derivatives thereof and methods of using same as fluorescent reagents.</p> |
