发明名称 |
<i>IN VIVO</i> SELECTION METHOD FOR DETERMINING INHIBITORY RNA MOLECULES |
摘要 |
A selection system suitable for use <i>in vivo</i> is provided, the system comprising: i) a plurality of first nucleotide sequences encoding a gene product capable of binding to and effecting the cleavage, directly or indirectly, of a nucleotide sequence, or a transcription product thereof; wherein a region of the first nucleotide sequence required for binding to the nucleotide sequence is heterogeneous within the plurality of first nucleotide sequences; and ii) a second nucleotide sequence comprising:(a) a coding region encoding a detectable marker operably linked to sequences required for mRNA stability and/or translation; and (b) a third nucleotide sequence positioned between the coding region and at least one of the sequences required for mRNA stability and/or translation; wherein (a) and (b) are operably linked to a regulatory sequence capable of directing expression of (a) and (b) as a contiguous RNA molecule in a host cell.
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申请公布号 |
WO0075370(A1) |
申请公布日期 |
2000.12.14 |
申请号 |
WO2000GB02136 |
申请日期 |
2000.06.02 |
申请人 |
OXFORD BIOMEDICA (UK) LIMITED;MITROPHANOUS, KYRI;KIM, NARRY;KOTSOPOULOU, EKATERINI |
发明人 |
MITROPHANOUS, KYRI;KIM, NARRY;KOTSOPOULOU, EKATERINI |
分类号 |
C12N15/09;C12N7/00;C12N7/01;C12N15/867;C12Q1/68;(IPC1-7):C12Q1/68;C12N15/86 |
主分类号 |
C12N15/09 |
代理机构 |
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主权项 |
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地址 |
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