| 摘要 |
<p>Provided is a polynucleotide comprising mRNA, rRNA or viral RNA, greater than 25 % of the ribose rings of which are covalently modified at the 2'-OH position. Further provided is a method for producing a double-stranded oligo- or polynucleotide from a template, which comprises contacting the template with a plurality of mononucleotides comprising UTP, dTTP and/or dUTP, ATP and/or dATP, GTP and/or dGTP, and CTP and/or dCTP, in the presence of a nucleic acid polymerase and optionally a template primer under conditions to polymerise the mononucleotides to form a nucleic acid strand complementary to the template, wherein the template comprises an oligo- or polyribonucleotide, a proportion of the ribose rings of which are covalently modified at the 2' -OH position to bear a substituent which enables replication of the template by the nucleic acid polymerase. Also provided is use of a poly-nucleotide comprising mRNA, rRNA or viral RNA, a proportion of the ribose rings of which are covalently modified at the 2' -OH position, in a hybridisation reaction.</p> |
