| 摘要 |
The invention provides a method of identifying an MCH receptor agonist or antagonist, by contacting an MCH receptor with one or more candidate compounds under conditions wherein the MCH receptor produces a predetermined signal in response to an MCH receptor agonist, and identifying a compound that alters production of the predetermined signal. The invention also provides a method of identifying an MCH receptor ligand, by contacting an MCH receptor with one or more candidate compounds under conditions that allow selective binding between the MCH receptor and an MCH receptor ligand, and identifying a compound that selectively binds to the MCH receptor. Also provided are methods of identifying an individual having or susceptible to an MCH receptor-associated condition, by detecting MCH receptor nucleic acid or polypeptide in a sample. The invention further provides signaling compositions, which contain a recombinantly expressed MCH receptor and a recombinantly expressed G alpha subunit of a G protein, or which contain a recombinantly expressed MCH receptor, a G protein, and a calcium indicator.
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