Method and compositions for stabilizing unstable gene transcripts

摘要

PCT No. PCT/US98/07691 Sec. 371 Date Oct. 15, 1999 Sec. 102(e) Date Oct. 15, 1999 PCT Filed Apr. 17, 1998 PCT Pub. No. WO98/48004 PCT Pub. Date Oct. 29, 1998A polynucleotide molecule useful for stably producing a gene product includes a polynucleotide sequence encoding the gene product (or alternatively a gene transcript) flanked by a 5' sequence and a 3' of an intron. Methods and compositions using this molecule permit enhanced recombinant expression of the gene product and are particularly useful in stabilizing unstable mRNA transcripts, permitting the stable production of desirable genes encoded thereby. Vectors and host cells containing this molecule are useful in the methods.