| 摘要 |
PCT No. PCT/JP96/01844 Sec. 371 Date Jan. 26, 1998 Sec. 102(e) Date Jan. 26, 1998 PCT Filed Jul. 3, 1996 PCT Pub. No. WO97/05246 PCT Pub. Date Feb. 13, 1997DNA strands having the ability to biotechnologically produce glycerol-3-phosphate acyltransferase (ATase) useful for converting the property of the PG of membrane lipids into that of more chilling resistance, specifically a chimeric gene of glycerol-3-phosphate acyltransferase (ATase) cDNA derived from squash in which the about one-third central region (the site cleaved by Kpn I and Hind III) has been replaced with the corresponding region of spinach ATase cDNA, a cDNA derived from squash in which the about one-sixth central region (the site cleaved by Hind III and Sac I) has been replaced with the corresponding region of spinach ATase cDNA, or a chimeric gene of ATase cDNA derived from spinach in which the about one-third 3'-terminal region (the site cleaved by Kpn I and Eco RI) has been replaced with the corresponding region of squash ATase cDNA are disclosed. These chimeric genes can express a chimeric ATase which has a higher substrate selectivity to unsaturated fatty acids. The DNA strand is introduced and expressed in a chilling sensitive plant, so that it can afford a plant the chilling resistance higher than that afforded by the ATase gene derived from a known chilling resistant plant.
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