| 摘要 |
<p>The invention relates to reporter molecules comprising: 1) at least one detection domain capable of emitting a signal; 2) at least one membrane anchoring domain, which promotes the association of the reporter molecule with a membrane; and at least one high specificity protease recognition site, which may be cleavable by a caspase. Proteolysis of the reporter molecule at a high specificity protease recognition site elicits or alters a signal from the detection domain. Consequently, the reporter molecules of the invention are useful for detecting or determining high specificity protease activity and may be applied to methods of drug discovery, for phenotypic evaluation of cells, including patient cells, and for tailoring a therapeutic program for treating a patient. Moreover, because caspases are intimately involved with programmed cell death, some embodiments of the invention are useful for monitoring apoptosis and effectors thereof.</p> |
