| 摘要 |
An enzyme exhibiting endo-~-1,4-glucanase activity which belongs to family 9 of glycosyl hydrolases is obtainable from or endogeneous to a strain belongi ng to the genus Bacillussuch as Bacillus licheniformis, ATCC 14580; an isolated polynucleotide (DNA) molecule encoding an enzyme or enzym e core (the catalytically active domain of the enzyme) exhibiting endo-~-1,4- glucanase activity selected from (a) polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 76 to nucleotide 1455 or from nucleotide 76 to nucleotide 1941, (b) polynucleotide molecules that encode a polypeptide being at least 75% identical to the amino acid sequence of SEQ ID NO:2 from amino acid residue 26 to amino acid 485 or from amino acid residue 26 to amino acid residue 646, and (c) degenerate nucleoti de sequences of (a) or (b), the expressed endoglucanase enzyme being useful in various industrial applications.
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