CHEMICAL ACTIVATION MEDIATED BY THE TRANSFER OF FLUORESCENT ENERGY FOR ELUCIDATING THE 3-D STRUCTURE OF BIOLOGICAL MACROMOLECULES

摘要

The present invention relates to a method for the selected chemical activation of photo-activatable cross-linker molecules around ligand binding pockets and fluorescent groups in macromolecules, especially biological macromolecules, by using fluorescent ligands of the macromolecule and by selecting photo-activatable cross-linker molecules having specific activation energies so that a radiationless energy transfer (Förster transfer) from the fluorescent ligands to the cross-linker molecules, which become activated thereby, takes place.