| 摘要 |
The present invention relates to a method for isolating fetal cells from maternal blood, wherein the blood sample has not been substantially enriched, such as by performing, on a sample of maternal blood from which at the most 50 % of the anucleated material cells thereof and/or at the most 50 % of the nucleated maternal cells thereof have been removed, selective labelling of fetal cells in the maternal blood sample, identifying the selectively labelled fetal cells, and specifically isolating substantially only the selectively labelled fetal cells. The selective labelling may be conducted with antibodies specific for the fetal cells and/or probes hybridising for example with fetal mRNA. Also, the invention relates to a method of diagnosing a disease in a fetus comprising obtaining a blood sample from the woman pregnant with said fetus, whereby at most 50 % of the maternal nucleated cells have been removed and/or at most 50 % of the maternal anucleated cells have been removed from said blood sample, selective labelling the fetal cells in the maternal blood sample, identifying the selectively labelled fetal cells, specifically labelling with at least one disease marker the identified fetal cells for diseases, and identifying specifically labelled cells. The specifically labelling may then be conducted with a marker, such as a probe, to a gene or a gene mutation specific for the genetic disease or a chromosome abnormality to be diagnosed.
|
