| 摘要 |
<p>The application and success of combinatorial approaches to protein engineering problems has increased dramatically. However, curren directed evolution strategies lack combinatorial methodology for creating libraries of hybrid proteins which lack high homology or for creating libraries of highly homologous genes with fusions at regions of non-identity. We have developed a series of combinatorial approaches that utilize the incremental truncation of genes, gene fragments or gene libraries to create such hybrid protein libraries. A library of all possible single base-pair deletions of a given piece of DNA is created. Incremental truncation libraries (ITLs) (as depicted in the figure) have applications in protein engineering as protein folding, protein evolution, and the chemical synthesis of proteins. In addition, a methodology of shuffling ITL's which is independent of DNA sequence homology has been developed.</p> |
