| 摘要 |
PROBLEM TO BE SOLVED: To carry out the mass pure culture of a wild strain of bdellovibrio in good efficiency, or carry out the mass culture by precisely purely isolating the bdellovibrio to be cultured. SOLUTION: A pure culture strain of purely isolated bdellovibrio live germ is aseptically inoculated to a sterilized liquid having suspended live bacteria or killed bacteria as host bacteria obtained by pure culture, and regulated salt concentration, and the inoculated germs are cultured under an aerobic condition. The bdellovibrio obtained by the pure isolation is used as the seed germ. When carrying out the pure culture, the antibodies formed by using contaminated microorganisms without the host bacteria of the bdellovibrio as antigens are added to the liquid containing the bdellovibrio and the other microorganisms to deactivate the contaminated microorganisms, and diluting the resultant liquid stepwise to provide purely isolated strain of the bdellovibrio.
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