| 摘要 |
PCT No. PCT/CA97/00175 Sec. 371 Date Jan. 5, 1999 Sec. 102(e) Date Jan. 5, 1999 PCT Filed Mar. 13, 1997 PCT Pub. No. WO97/34003 PCT Pub. Date Sep. 18, 1997The invention provides DNA isolated from a plant species of the family Brassicaceae that can be introduced into the genomes of plants to produce genetically-modified plants having higher levels of squalene than the natural plants. The DNA corresponds to squalene epoxidase gene of the same or a related plant, and may have the sequence as shown by SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5, or a sequence having at least 60% identity with such a sequence. The DNA is introduced into the genome in a way that results in down-regulation of an exogenous plant squalene gene to suppress the expression of squalene epoxidase. The invention also relates to a process of producing genetically-modified plants, plasmids and vectors used in the method, genetically-modified plants and seeds thereof and a method of producing squalene from the modified plants.
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