PIEZOELECTRIC QUARTZ CRYSTAL SENSOR FIXING THIOLATED ANTIBODY FOR DETECTING FOOD POISONING BACTERIA, AND PREPARATION METHOD THEREOF

摘要

PURPOSE: A piezoelectric quartz crystal sensor for detecting food poisoning bacteria and its preparation method are provided, to improve the sensitivity and the stability. CONSTITUTION: The method comprises the steps of dissolving antibacterial antibody against food poisoning bacteria in standard buffering solution in 0.1-7 mg/ml; making 10-70 mM of thiolated cross-linking solution; putting the two solution test tube and reacting the mixture at 10-50°C for 10-300 min; adding dithiothreitol to the test tube and reacting it for 10-200 min; coating the one surface of gold electrode of pre-activated piezoelectric quartz crystal with the obtained thiolated antibacterial antibody solution; reacting it at 15-40°C for 10-300 min; drying it; and washing it with standard buffering solution and distilled water to prepare the piezoelectric quartz crystal sensor for detecting food poisoning bacteria.