Thermostable nucleic acid polymerase with 3'-5' proofreading exonuclease ability from Thermococcus gorgonarius

摘要

A purified thermostable enzyme is derived from the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90 % of its activity after incubation for two hours at 95oC in the presence of stabilising agents and possesses 3'-5' proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).