E1 ENDOGLUCANASE VARIANTS Y245G, Y82R AND W42R

摘要

The invention provides a method for making a glycosyl hydrolase characterize d by an increase in catalytic activity on an insoluble substrate, comprising replacing an active site associated glycosyl-stabilizing amino acid of the hydrolase with an amino acid, the replacing amino acid not strongly binding a disaccharide product in the active site, and a method for making a glycosyl hydrolase characterized by an increase in catalytic activity on a soluble substrate, comprising replacing a hydrophobic substrate binding amino acid o f the hydrolase with a positively charged amino acid. The invention further provides a glycosyl hydrolase, comprising Y42R (SEQ. ID NO:1), W82R (SEQ. ID NO:2), or Y245G (1) (SEQ. ID NO:3) and the DNA sequences encoding the enzyme s.