发明名称 |
METHOD OF PREPARING HYALURONIDASE |
摘要 |
natural-origin biologically- active substances. SUBSTANCE: in a method of preparing preparation of testicular hyaluronidase, testes of slaughtered livestock are ground and homogenized. Homogenate is extracted with acetic acid at weight/volume ratio 1:1.5, frozen at temperature from -5 to -20 C, thawed, and concentrated involving ultrafiltration technique in tangential flow on membranes providing selectivity 15-20 kD. Product is purified by consecutive excluding sorption of ballast ethacrydine impurities using lactate and activated carbon. EFFECT: increased yield and activity enzyme preparation. 1 tbl, 2 ex
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申请公布号 |
RU2159284(C1) |
申请公布日期 |
2000.11.20 |
申请号 |
RU19990112225 |
申请日期 |
1999.06.07 |
申请人 |
GOSUDARSTVENNOE UNITARNOE PREDPRIJATIE "IMMUNOPREP;GUP IMMUNOPREP |
发明人 |
JAKIN O.G.;MEL'NIKOV N.V.;KOZYREVA M.KH.;VAKHITOV R.Z.;NIGAMOV F.N.;VAKHITOV R.SH.;JUSUPOV V.G. |
分类号 |
C12N9/26;A61K35/48;(IPC1-7):C12N9/26 |
主分类号 |
C12N9/26 |
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