| 摘要 |
There are provided a method of determining a genotype of CYP2A6 gene on the basis of a difference in gene structures of a wild-type gene of CYP2A6 and at least one kind of mutant-type gene associated with mutation of CYP2A6 gene; the method mentioned above, wherein one kind of mutant-type gene associated with mutation of CYP2A6 gene is used, and the mutant-type gene is conversion-type CYP2A6 gene comprising the nucleotide sequence of SEQ ID NO: 4; the method mentioned above, wherein homozygous deletion and heterozygous deletion of CYP2A6 gene are determined by using at least two kinds of mutant-type genes associated with mutation of CYP2A6 gene; a method of determining a genotype of a CYP gene, characterized by carrying out a method comprising the method mentioned above to determine the genotype of the CYP gene; a diagnostic kit for determining a genotype of CYP2A6 gene, comprising 5'-primer capable of hybridizing to both exon 8 of CYP2A6 gene and exon 8 of CYP2A7 gene, 3'-primer capable of specifically hybridizing to exon 9 of CYP2A6 gene, AccII or an isoschizomer thereof, and Eco81I or an isoschizomer thereof; and the like. |
