PURIFICATION METHOD BY AFFINITY

摘要

The invention concerns a novel method for producing and purifying a protein or a polypeptide of interest by affinity chromatography on a solid support bearing sulphated polysaccharides or functional analogues and comprising the grafting upstream or downstream of said polypeptide of interest with a new molecular label consisting of at least one R1 motif of formula X1X2Y1Y2PY3 between 0 and five R2 motifs of formula X1X2Y1Y2Y3X3X4 wherein X1 to X4 represent indifferently K(Lys) or R(Arg), and Y1 to Y3 represent any amino acid, but preferably A(Ala), L(Leu), I(IIeu), V(Val) and P represents a proline, the number of R1 and R2 motifs and their combination being selected on the basis of the desired affinity between the polypeptide and the solid support bearing sulphated polysaccharides, provided that when the number of R2 is equal to 0, the number of R1 is at least equal to 2.