METHODS FOR CONSTRUCTING DNA LIBRARY AND SUPPORT CARRYING DNA LIBRARY IMMOBILIZED THEREON

摘要

A method for constructing a cDNA library which comprises hybridizing mRNA with oligo(dT)n on a support and treating with a reverse transcriptase so as to immobilize complementary DNA; or a method for constructing a gDNA library which comprises ligating a double-stranded chromosomal DNA library with an oligonucleotide on a support having a restriction enzyme site and then immobilizing the gDNA library; a method for preparing replicas thereof; and a support carrying the thus replicated DNA fragment thereon.