发明名称 |
METHODS FOR CONSTRUCTING DNA LIBRARY AND SUPPORT CARRYING DNA LIBRARY IMMOBILIZED THEREON |
摘要 |
A method for constructing a cDNA library which comprises hybridizing mRNA wi th oligo(dT)n on a support and treating with a reverse transcriptase so as to immobilize complementary DNA; or a method for constructing a gDNA library which comprises ligating a double-stranded chromosomal DNA library with an oligonucleotide on a support having a restriction enzyme site and then immobilizing the gDNA library; a method for preparing replicas thereof; and a support carrying the thus replicated DNA fragment thereon.
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申请公布号 |
CA2372593(A1) |
申请公布日期 |
2000.11.16 |
申请号 |
CA20002372593 |
申请日期 |
2000.05.10 |
申请人 |
TOYO KOHAN CO., LTD.;NIHON PARKERIZING HIROSHIMA CO., LTD. |
发明人 |
TAKAI, OSAMU;TAKAHASHI, KOJIRO;TANGA, MICHIFUMI |
分类号 |
C12N15/10;(IPC1-7):C12N15/00;C12M1/34;C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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