DETECTION OF PATHOGENS, ESPECIALLY SPIROCHETES, FROM BODY FLUIDS

摘要

A reagent for microscopy-based detection of pathogens, especially spirochetes, from body fluids, characterised by that containing the following ingredients: tetracaine (125-200 mg/l), mannit (1500-2000 mg/l), EGTA (etilene bis[oxyetiline-nitrilo]-tetraacetate), 0,76-0,114 mg/l magnesium-chloride (preferably in an amount of 0,10 mg/l), caffeine-sodium-benzoate (2000-4000 mg/l), glucose (1800-2200 mg/l), glycerol 75-105 mg/l), optimally tri-sodium-citrate (preferable in an amount of 10000 mg/l), Hoechts 33343 dye (1,11 mg/l) is required 20 to 40 ml of distilled water and RPMI 1640 culture media to make 100 ml. The invention also included the pathogen detection procedure.