MASS PRODUCTION METHOD OF SOMATIC EMBRYO AND SEEDLING BY SUSPENSION CULTURING OF PANAX GINSENG/EMBRYOGENIC CELLS, AND USE THEREOF

摘要

PURPOSE: A mass production method of somatic embryo and seedling by suspension culturing of Panax ginseng/Embryogenic cells, and use thereof in health food or pharmaceutical preparation and ginseng seedlings for raising are provided. CONSTITUTION: A process for the mass production of Panax ginseng/Embryogenic cells comprises of: sterilizing the albumen of ginseng seed; washing with sterilized water for 3times; culturing at MS(Murashige and Skoog) agar plate; after 4weeks, compact embryogenic callus is induced; culturing the embryogenic callus at MS agar plate to get friable embryogenic callus; selecting the friable embryogenic callus, and suspension culturing at MS liquid medium for 3weeks to get large quantity of embryogenic cells. A process for the mass production of somatic embryo and seedling comprises of: culturing the embryogenic cells at MS liquid medium for 3weeks under fluorescence light to get globular shaped embryo; and culturing successively to separate somatic embryo and seedling from Panax ginseng/Embryogenic cells.