Method for the detection of viable Cryptosporidium parvum oocysts

摘要

PCT No. PCT/AU96/00274 Sec. 371 Date Mar. 3, 1998 Sec. 102(e) Date Mar. 3, 1998 PCT Filed May 6, 1996 PCT Pub. No. WO96/34978 PCT Pub. Date Nov. 7, 1996Oligonucleotide molecules and methods are disclosed for the detection of viable oocysts or other cells of the protozoa species, Cyrptosporidium parvum. Preferred oligonucleotide molecules are selected from the group comprising oligonucleotides having one or more of the following sequences: (a) ACA ATT ATT, (b) CTT TTT GGT, (c) ATT TTA TAT AAA ATA TTT TGA TGA A, (d) TTT TTT TTT TTA GTA T, (e) TAT ATT TTT TAT CTG, (f) CTT TAC TTA CAT GGA TAA CCG, or comprising a part of the sequences (a) to (f) above so as to allow specific hybridization to unique 18S rRNA sequences of C. parvum.