发明名称 |
Green fluorescent protein |
摘要 |
PCT No. PCT/US94/10165 Sec. 371 Date Jun. 18, 1996 Sec. 102(e) Date Jun. 18, 1996 PCT Filed Sep. 9, 1994 PCT Pub. No. WO95/07463 PCT Pub. Date Mar. 16, 1995This invention provides a cell comprising a DNA molecule having a regulatory element from a gene, other than a gene encoding a green fluorescent protein operatively linked to a DNA sequence encoding the green fluorescent protein. This invention also provides living organisms which comprise the above-described cell. This invention also provides a method for selecting cells expressing a protein of interest which comprises: a) introducing into the cells a DNAI molecule having DNA sequence encoding the protein of interest and DNAII molecule having DNA sequence encoding a green fluorescent protein; b) culturing the introduced cells under conditions permitting expression of the green fluorescent protein and the protein of interest; and c) selecting the cultured cells which express green fluorescent protein, thereby selecting cells expressing the protein of interest. Finally, this invention provides various uses of a green fluorescent protein.
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申请公布号 |
US6146826(A) |
申请公布日期 |
2000.11.14 |
申请号 |
US19960367236 |
申请日期 |
1996.06.18 |
申请人 |
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK;WOODS HOLE OCEANOGRAPHIC INSTITUTION |
发明人 |
CHALFIE, MARTIN;PRASHER, DOUGLAS |
分类号 |
G01N33/48;C07K14/435;C12N1/15;C12N1/21;C12N5/10;C12N15/09;C12N15/65;C12P21/02;C12Q1/02;C12Q1/68;G01N33/18;G01N33/58;(IPC1-7):C12Q1/68 |
主分类号 |
G01N33/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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