发明名称 |
Synthetic gene for expressing active retroviral protein in eukaryotes |
摘要 |
The present invention features a synthetic gene or region of a gene which has an amended codon usage compared with the wild-type gene and which is for the high level expression of a retroviral protein in eukaryotic cells, the expressed retroviral protein having enzymatic activity in the eukaryotic cell. In addition, the invention features a synthetic gene or region of a gene encoding a retroviral enzyme or part of a retroviral enzyme normally expressed in a mammalian or other eukaryotic cell wherein at least one non-preferred codon in the wild-type gene encoding the enzyme has been replaced by a preferred codon encoding the same amino acid. The retroviral protein may be a protease, reverse transcriptase, integrase protein or a polyprotein gag-pol precursor thereof. In one embodiment the retroviral protein with enzymatic activity is a lentiviral protein. In other embodiments the enzymatically active protein is a pol enzyme. In more preferred embodiments, the enzymatically active protein is a lentiviral integrase. In an even more preferred embodiment the enzyme is an HIV enzyme. In more preferred embodiments the enzymatically active protein is HIV integrase. The present invention also includes a detection method for intracellular integrase using a promoterless reporter gene. |
申请公布号 |
AU4557900(A) |
申请公布日期 |
2000.11.10 |
申请号 |
AU20000045579 |
申请日期 |
2000.04.26 |
申请人 |
K.U. LEUVEN RESEARCH & DEVELOPMENT |
发明人 |
ZEGER DEBYSER;ERIK DE CLERCQ;PETER CHEREPANOV;WIM PLUYMERS |
分类号 |
A01K67/027;A61K48/00;C07K14/16;C12N5/10;C12N7/04;C12N15/00;C12N15/09;C12N15/49;C12N15/86;C12N15/867;C12Q1/25 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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