A METHOD FOR MEASURING BIOMOLECULES

摘要

This present invention is for a method referred to as the vessel transfer method that greatly simplifies the detection and determination of the identity, amount and activity of molecules with biological activity. It is concerned with use of a vessel that will facilitate and simplify the measurement of the amount and activity of enzymes, enzyme inhibitors, lectins, receptors and other biologically active substances using a vessel transfer for isolating a reactant from a product after completion of the reaction. The method involves a surface is coated with a reactant, the appropriate compounds are added, allowing the reaction to proceed for a given period of time and then reaction solution is transferred from the reaction vessel 1 coated with a reactant to the vessel 2 whose surface is not coated. The amount of labeled compounds in the vessel 2 can be directly measured.