(gluconobacter suboxydans) sorbitol dehydrogenase, genes and methods of use thereof

摘要

The invention relates to the fields of molecular biology, bacteriology and industrial fermentation. More specifically, the invention provides isolated nucleic acid molecules encoding the three subunits of a novel, membrane-bound, Gluconobacter oxydans sorbitol dehydrogenase (SDH) of the invention and vectors and host cells containing said isolated nucleic acid molecules. The invention further provides isolated polypeptides for the three subunits ofthe SDH enzyme of the invention, and processes for the production of L-sorbose and 2 keto-L-gulonic acid.