发明名称 |
OPTICAL PROBES AND ASSAYS |
摘要 |
This invention provides an optical probe useful as an optical probe or senso r of post translational type modifications, such as phosphorylation. The invention comprises a polypeptide moiety, which contains a recognition motif for a post translational type activity, and a protease site, which is couple d to a probe moiety. Modification of the polypeptide, by the post translationa l type activity, results in a modulation of the rate at which a protease cleav es the polypeptide which is sensed by a measurable change in at least one optic al property of the optical probe upon cleavage. The present invention also includes a recombinant nucleic acid molecule that encodes an optical probe a nd a vector and host cell or library of cells that include the recombinant nucleic acid molecule. The optical probe can be used in methods to determine whether a sample, including a cell or a sample from an organism, contains a post-translational type modification activity. Such methods can also be used to determine whether a test chemical modulates the activity of a modifying activity, and thus can be used to identify therapeutic compositions. The identification of such therapeutic compositions can be automated using a system that includes an optical probe.
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申请公布号 |
CA2370111(A1) |
申请公布日期 |
2000.11.09 |
申请号 |
CA20002370111 |
申请日期 |
2000.05.05 |
申请人 |
AURORA BIOSCIENCES CORPORATION |
发明人 |
POLLOK, BRIAN A.;RODEMS, STEVEN M.;MAKINGS, LEWIS R.;HAMMAN, BRIAN D. |
分类号 |
G01N33/483;A61K45/00;A61P43/00;C07K14/435;C12M1/00;C12M1/34;C12N15/09;C12Q1/37;C12Q1/42;C12Q1/48;C12Q1/68;G01N21/78;G01N27/447;G01N33/542;G01N37/00;(IPC1-7):C12Q1/37 |
主分类号 |
G01N33/483 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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