PROCESS FOR PRODUCING RECOMBINANT INSULIN FROM NOVEL FUSED PROTEIN

摘要

A DNA encoding a fused protein of formula (I) comprising a leader peptide sequence (Y) consisting of at least one amino acid residue for the expression and secretion of the protein, an amino acid sequence (X1) to be used in enzymatic or chemical cleavage, an amino acid sequence (B-chain) of insulin B chain, an amino acid sequence (X2) to be used in enzymatic cleavage, a linker sequence (Linker) consisting of at least one amino acid residue, an amino acid sequence (X3) to be used in enzymatic cleavage, and an amino acid sequence (A-chain) of insulin A chain, which are linked to each other in this order; and a process for producing insulin by using an expression system containing this DNA: [Y]-[X1]-[B-chain]-[X2]-[Linker]-[X3]-[A-chain] (Formula 1). Thus, a process for efficiently producing gene recombinant insulin at a high productivity is provided.