EXPRESSION OF HEPARIN-BINDING PROTEIN IN RECOMBINANT MAMMALIAN CELLS

摘要

The present invention relates to a method for producing a mammalian heparin- binding protein in a mammalian cell that can be cultured under anaerobic conditions after introducing a nucleic acid encoding as heparin-binding protein into said cell with comprising (a) introducing into said mammalian cell a nucleic acid encoding said heparin-binding protein; (b) culturing the cell of step (a) under conditions conducive to expression of said HBP; and ( c) recovering said HBP from the culture medium. Furthermore, the invention relates to the recombinant mammalian cell used in the method.