| 摘要 |
PCT No. PCT/JP97/03445 Sec. 371 Date Nov. 24, 1998 Sec. 102(e) Date Nov. 24, 1998 PCT Filed Sep. 26, 1997 PCT Pub. No. WO98/42857 PCT Pub. Date Oct. 1, 1998A method for transferring a long-chain DNA into protoplasts, comprising the steps of: (a) mixing a suspension of protoplasts, polyethylene glycol, and a long-chain DNA to give a final concentration of the DNA of at least 50 mu g/ml; (b) collecting, washing, and culturing the protoplasts in a medium; and (c) selecting transformed cells, is provided. By this gene transfer method, long-chain DNA can be conveniently transferred into a wide variety of plant cells.
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