| 摘要 |
PROBLEM TO BE SOLVED: To achieve a uniform system enzyme immunity analysis method that has a low background value and can secure sufficient S/N ratio by allowing a ligand, an enzyme labeled ligand, an antibody for the ligand, and a second antibody for the antibody to react one another and measuring the change in the enzyme activity of the labeled enzyme in a reaction liquid. SOLUTION: A ligand, an enzyme labeled ligand, an antibody for the ligand, and a second antibody for the antibody are allowed to react one another, and the change in the enzyme activity of a labeled enzyme in a reaction liquid is measured, thus measuring the amount of ligand. The ligand with an antigen determinant being contained in a specimen, namely an antigen, is to be measured. The specimen includes blood, lymph, and urine. The enzyme labeled ligand contains a substance that shares the ligand with a light source determinant being subjected to enzyme labeling. By combining the enzyme labeled ligand with the antibody and then combining the second antibody, steric hindrance for the labeled enzyme is increased. The enzyme activity of the labeled enzyme in the reaction liquid can be measured by adding an enzyme substrate to the reaction liquid.
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