IMMOBILIZED ENZYMES AS BIOSENSORS FOR CHEMICAL TOXINS

摘要

Methods, compositions and materials useful in the detection of organophosphorous and organosulfur are disclosed. In particular, biosensors wherein a porous e.g. foam or a non-porous support having an enzyme covalently immobilized upon or within are disclosed. The biosensors exhibit enzymatic stability at extreme temperatures and/or denaturing conditions, and similar kinetic characteristics of the soluble form of the enzymes utilized. The enzyme does not leach from the porous or non-porous support and the material retains enzymatic activity after prolonged storage. Differential biosensors are also disclosed.