发明名称 |
SYNTHETIC GENE FOR EXPRESSING ACTIVE RETROVIRAL PROTEIN IN EUKARYOTES |
摘要 |
The present invention features a synthetic gene or region of a gene which ha s an amended codon usage compared with the wild-type gene and which is for the high level expression of a retroviral protein in eukaryotic cells, the expressed retroviral protein having enzymatic activity in the eukaryotic cel l. In addition, the invention features a synthetic gene or region of a gene encoding a retroviral enzyme or part of a retroviral enzyme normally express ed in a mammalian or other eukaryotic cell wherein at least one non-preferred codon in the wild-type gene encoding the enzyme has been replaced by a preferred codon encoding the same amino acid. The retroviral protein may be a protease, reverse transcriptase, integrase protein or a polyprotein gag-pol precursor thereof. In one embodiment the retroviral protein with enzymatic activity is a lentiviral protein. In other embodiments the enzymatically active protein is a pol enzyme. In more preferred embodiments, the enzymatically active protein is a lentiviral integrase. In an even more preferred embodiment the enzyme is an HIV enzyme. In more preferred embodiments the enzymatically active protein is HIV integrase. The present invention also includes a detection method for intracellular integrase using a promoterless reporter gene. |
申请公布号 |
CA2369058(A1) |
申请公布日期 |
2000.11.02 |
申请号 |
CA20002369058 |
申请日期 |
2000.04.26 |
申请人 |
K.U. LEUVEN RESEARCH & DEVELOPMENT |
发明人 |
DEBYSER, ZEGER;PLUYMERS, WIM;CHEREPANOV, PETER;DE CLERCQ, ERIK |
分类号 |
A01K67/027;A61K48/00;C07K14/16;C12N5/10;C12N7/04;C12N15/00;C12N15/09;C12N15/49;C12N15/86;C12N15/867;C12Q1/25 |
主分类号 |
A01K67/027 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|