Disclosed herein are cultures of undifferentiated avian primordial germ cells/blastodermal cells. The cultures are capable of maintaining their undifferentiated characteristic and also their capability of expressing the EMA-1 epitope for extended periods when cultured in the presence of an isolate from avian navel (e.g. turkey navel extract). Methods of culturing such cultures using avian navel extracts, and culture media containing avian navel extract are also disclosed. Recombinant birds derived from these cultures are also disclosed. These cultures can be frozen for long-term preservation of germ line genomes.
申请公布号
US6140118(A)
申请公布日期
2000.10.31
申请号
US19990372702
申请日期
1999.08.11
申请人
WISCONSIN ALUMNI RESEARCH FOUNDATION;THE UNITED STATES OF AMERICA AS REPRESENTED BY THE DEPARTMENT OF AGRICULTURE
发明人
TSAI, HERNG;HUNT, HENRY D.;BACON, LARRY D.;WENTWORTH, BERNARD C.;WENTWORTH, ALICE L.