摘要 |
PROBLEM TO BE SOLVED: To provide a method for accomplishing a sterilization assurance having reproducibility in the case of carrying out a sterilization assurance related to a high-pressure steam sterilization defined by ISO 11, 134. SOLUTION: In carrying out a sterilization assurance related to a high- pressure steam sterilization defined by ISO 11, 134 by using a thermally damaged bacterium of Bacillus stearothermophilus being a biological indication bacterium, a solid medium or a liquid medium having >=80 mg/L Ca ion concentration in a medium is used. |