发明名称 |
RECOMBINANT PROTEIN PRODUCTION IN A HUMAN CELL USING SEQUENCES ENCODING ADENOVIRUS E1 PROTEIN |
摘要 |
The present invention provides methods and compositions for the production o f recombinant proteins in a human cell line, using sequences encoding at least one E1 protein of an adenovirus where the cells does not encode a structural adenoviral protein from its genome. The methods and compositions are particularly useful for generating stable expression of human recombinant proteins of interest that are modified post-translationally, e.g. by glycosylation. Such proteins may have advantageous properties in comparison with their counterparts produced in non-human systems like Chinese Hamster Ovary (CHO) cells.
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申请公布号 |
CA2370477(A1) |
申请公布日期 |
2000.10.26 |
申请号 |
CA20002370477 |
申请日期 |
2000.04.17 |
申请人 |
CRUCELL HOLLAND B.V. |
发明人 |
HATEBOER, GUUS;BOUT, ABRAHAM;SCHOUTEN, GOVERT JOHAN;UYTDEHAAG, ALPHONSUS GERARDUS CORNELIS MARIA;VERHULST, KARINA CORNELIA |
分类号 |
C12N15/09;A61K39/125;A61K39/145;A61K39/21;A61K39/23;A61K39/245;A61K39/29;A61K39/395;A61K48/00;A61P31/12;C07K14/075;C07K14/505;C12N5/06;C12N5/10;C12N15/85;C12N15/86;C12P21/02;C12R1/91;(IPC1-7):C12N15/85 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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地址 |
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